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Normalization was carried out to account for technical variation, and keratins identified in blank Schirmer strip controls were removed, as previously published 30

A faint blue tint in the reconstituted GHK-Cu solution is characteristic of the copper(II) complex and directly indicates that the peptide is properly chelated with copper

Ratiometric image analysis was conducted using a Python script (available from The background was subtracted for each channel and the ROIs (=transfected cells) were determined by thresholding or with the Cellpose 40 algorithm in the channel of the FP

Using liver microsomes from human liver bank samples, three linked single nucleotide polymorphisms (SNPs) rs10929303, rs1042640, and rs8330 in the UGT1A-3UTR region were associated with acetaminophen glucuronidation [20]

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