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Standard assays of GST activity The activities of the GST stocks were assayed at 30 C with the standard substrates, 1 mM CDNB (stock solution in ethanol) and 1 mM GSH in 0.1 M sodium phosphate buffer pH 6.5, containing 1 mM EDTA, and the formation of the product S-2,4-dinitrophenyl-glutathione (DNP-SG) was followed spectrophotometrically at 340 nm 23

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Hemmati, F., Akinpelu, A., Song, J., Amiri, F., McDaniel, A., McMurray, C., et al

doi: 10.1038/s41598-017-18079-2

Finally, an approach could be drawn where skin diseases and ageing will be prevented by using pycnogenol administration