doi: 10.3390/ijms21030776 49 CorralizaIMSolerGEichmannKModolellM
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This is a state where there is too much acid in the body fluids
For reverse transcription (RT), 2 g of total RNA was mixed with 1 L Oligo d(T)15 primer (0.5 g/L) and water pretreated with diethylpyrocarbonate (DEPC-H 2 O), and the mixture was incubated for 5 min at 70 C
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