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In addition, a marked decrease in body weight was seen with tirzepatide ranging from 0.9 to 11.3 kg, and a summary of the weight reduction for each clinical trial is shown in Figure 4

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The partially purified proteins in 10 kDa spin columns were desalted by washing twice with ice-cold buffer (25 mM MES [pH 6.0], 150 mM NaCl, 5% glycerol, 0.4% (w/v) n-Nonyl--d-glucopyranoside) and concentrated to about 10 mg ml 1 followed by loading onto a Superdex-200 gel filtration column (GE Healthcare Life Sciences, PA) to remove the contaminated proteins

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Mandal, Pravat K., et al