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To generate pEGFP-elt-3 , pEGFP-pqm-1 , and pEGFP-hmp-2 , elt-3 , pqm-1 , and hmp-2 cDNA were amplified from N2 cDNA and cloned at the C terminal, N terminal, and N terminal of EGFP in pEGFP-c1 , respectively