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They were cultured, respectively in Dulbecco's Modified Eagle Medium, Iscove's Modified Dulbecco's Medium, and Roswell Park Memorial Institute 1640 medium (Hyclone) with 10% fetal bovine serum (Gibco) under incubation at 37C with 5% carbon dioxide

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It is slightly more complex than using a single vial size throughout, but it optimizes both measurement precision and syringe convenience at every stage

Thus, the extent to which endometriosis causes oocyte aneuploidy remains to be answered definitively, and if it does, the extent to which the aneuploidy is caused by ROS in endometriosis will need to be addressed

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