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The eluted sample was quickly neutralized by addition of 1/10 volume of 1 M Tris pH 8.0 and further polished on a size-exclusion chromatography column (Superdex 75, GE healthcare) pre-equilibrated with phosphate-buffered saline (PBS, pH 7.4)

The authors suggest that FFA3 might act to prevent hyperphagia-induced over-secretion of insulin that would cause beta cell exhaustion during dietary-related stress (Priyadarshini et al., 2020)

Benefits of GLP-1 Medications Assisted Weight Loss: Clinical studies have shown that GLP-1 medications can facilitate effective weight loss in individuals with obesity

Some patients also benefit from creatine supplementation, which has an excellent safety profile and supports muscle maintenance

These effects promote weight loss and improve glycemic control