For antibody labeling, after treatments at 37 C, cells were placed on ice to arrest further endocytosis before fixation, and the surface GLP-1R was detected by ELISA with monoclonal anti-human GLP-1R antibody 68 (Mab 3F52, Developmental Studies Hybridoma Bank (DSHB), 1/100) plus horseradish peroxidase (HRP)-conjugated rabbit anti-mouse secondary (ab97046, Abcam, 1/5,000)
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Any spotting experienced during treatment is more likely related to weight loss itself rather than a direct pharmacological effect
Second, by improving insulin sensitivity, these medications may help mitigate the established link between insulin resistance and endometriosis severity
The right pathway depends on your health history, BMI, metabolic conditions, lifestyle and long-term goals
