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After filtration with 200 mesh screen and centrifugation at 1500 rpm, the precipitates were inoculated in Dulbeccos modified Eagles medium (DMEM) complete medium containing 1% penicillinstreptomycin and 10% FBS and cultured at 37C and 5% CO 2 saturation humidity for 1.5 h, and then, the nonadherent cells were collected

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They also presented data suggesting the presence of putative synaptic markers in GLP-1 and enterochromaffin cells 39,58,59 , as well as viral tracing indicating synaptic-like connections with a small number of vagal afferents 38,59

Fifth, the lack of detailed clinical parameters limited our ability to perform formal risk stratification based on liver disease severity

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