Insert the needle into the vial with powder and inject sterile water into the vial slowly until all powder is dissolved usually takes about 5 seconds
The FDA's Category 2 designation means the substance may present significant safety risks when used in compounding and is prohibited in compounded medications pending further evaluation
While B12 is naturally found in foods like meat, eggs, and dairy, some individuals have difficulty absorbing enough through diet alone
Reyes-Prieto and Moustafa, 2012
Essential Equipment and Materials Required Equipment: Sterile glass vials or low-binding microcentrifuge tubes Calibrated micropipettes (10-1000 L range) Sterile filter tips Vortex mixer or gentle rocker Analytical balance (0.1 mg precision) Reconstitution Solvents: Sterile water (molecular biology grade) Bacteriostatic water (contains 0.9% benzyl alcohol as preservative) Phosphate buffered saline (PBS, pH 7.4) Dilute acetic acid solution (0.1% v/v) DMSO (for highly hydrophobic peptides) Optional Materials: Sonicator bath pH meter 0.22 m sterile filters All materials must be sterile and free from endotoxins for cell culture applications
An intradermal test dose is recommended before Cyanocobalamin Injection, USP is administered to patients suspected of being sensitive to this drug
