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A caloric deficit triggers tissue breakdown for energy, affecting both fat stores and muscle protein

We also assess your current weight, BMI, and any previous attempts at weight loss through diet and exercise

The mice were euthanized at 2 h after administration, and the brains were carefully collected, dehydrated, embedded, sectioned, and transferred to microscope slides

# 714003, MilliporeSigma) cultured in LB medium with 50 g/mL kanamycin and 25 g/mL chloramphenicol