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Simple Western assay for protein expression HJEs were scraped into lysis buffer (Tris HCl 20 mM pH 7.4, NaCl 150 mM, EDTA 5 mM, Triton X-100 1% v/v, sodium deoxycholate 0.5% w/v) supplemented with Protease Inhibitor Cocktail (Sigma)

How it works: GLP-1 and GIP suppress appetite, slow digestion, and enhance insulin secretion

complete phase III trials of novel therapies (e.g., targeting mitochondrial function, cellular senescence, microbiome) and facilitate their clinical translation

Abstract L-carnitine, available as feed additive, is essential for the beta-oxidation of free fatty acids in the mitochondrial matrix

For maximum anti-aging effect, GHK-Cu is superior

Notably, improvements in TAC/MDA occur even when glycemic indices are unchanged, implying a primary redox effect that may precede metabolic reprogramming