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The cell pellets were thawed and lysed in a buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 10% (v/v) glycerol, 10 mM MgCl 2 , 1 mM MnCl 2 and 100 M TCEP supplemented with EDTA-free protease inhibitor cocktail (Bimake) by dounce homogenization

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Feb 1 2010;34(1):76-80

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