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The neutralization titers were defined as the highest serum dilutions that inhibited at least 90% of GFP fluorescent focus formation

MPTP is an alipophilic prototoxin that rapidly crosses the blood-brain barrier and damage dopaminergic neurons due to the selective uptake of the active metabolite MPP + via the dopamine transporter [80]

Marshall et al., 2011)

Ni-NTA resin (2 mL), pre-equilibrated with 20 mM imidazole (all buffer contained 150 mM NaCl and 20 mM HEPES pH 7.5), was added into the supernatant for batch binding with stirring at 4 C for 1.5 h