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This follow-up time is established based on the remaining activity of gluten and its bio products in patients with celiac disease after elimination of this protein from the diet [1]

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Briefly, plates were coated with Bet v 1 and incubated with mouse sera for 2 hours at room temperature, followed by incubation with HRP-conjugated goat anti-mouse IgE antibodies (#PA184764, Thermo Fisher, USA, 1:1000)

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