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Bone marrow chimeras were generated by lethally irradiating Dpp4 EC+/+ (pool of Dpp4 fl/fl and Tie2 -Cre mice) and Dpp4 EC/ mice (1100 cGy split into 2 equal doses 4 hours apart), followed by reconstitution with 5 10 6 bone marrow cells from donor B6.SJL-Ptprc a Pepc b /BoyJ mice as described previously (56)
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To be considered for treatment, clients must meet at least one of the following criteria: A BMI of over 30kg/m A BMI of over 27kg/m with at least one weight-related condition, which includes: Type 2 diabetes Hypertension High cholesterol Who should NOT take Semaglutide
[34] Low-retention tips and calibrated micropipettes (or sterile transfer tools aligned with your SOP) [24] Alcohol prep (e.g., 70% IPA) for exterior wipe-down of vial stoppers and surfaces (per lab SOP) Labels for concentration, solvent, date/time, and storage condition Single-use aliquot tubes (recommended to avoid repeated freezethaw cycles) [34] Reconstitution Calculations: mg mL (Target Concentration) For research workflows, document concentration clearly (mg/mL or g/mL)
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