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In the DUAL II, DUAL V and Lixilan-L, Liraglutide and Lixisenatide were titrated depending on basal insulin requirement for obtaining fasting glucose control on the bases of a treat-to-target approach

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In the first protocol, growth factor-reduced matrigel was pipetted into prechilled 24-well plates (150 mL matrigel per well) and polymerized for 45 minutes at 37C