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The particles were recovered by centrifugation at 14,000 g for 15 min room temperature following freeze drying (Buchi, Lyovapor L-200)

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Conclusion: Conclusively, PA can be considered a potential compound that can attenuate acetaminophen-induced hepatotoxicity through its role as an iron chelator and antioxidant, as well as the up-regulation of SIRT -1 and down-regulation of CYP2E1

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Western blot results showed that GHK-Cu significantly upregulated SIRT1 expression in the colon tissues of DSS-induced mice and MCECs in the co-culture system