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40 g of protein lysates, unless otherwise indicated, were resuspended in Laemmli buffer (20% glycerol, 125 mM Tris-HCl, 4% sodium dodecyl sulfate, 10% -mercaptoethanol, 0.075% bromophenol blue) 131 , separated in a 12% SDS-polyacrylamide gel electrophoreses (SDS-PAGE), and electroblotted to nitrocellulose

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