When SAM is reduced as occurs downstream of MTHFR-impaired methionine regeneration DNA methyltransferase activity is substrate-limited, producing a pattern of progressive CpG methylation loss at sites that require continuous active maintenance
SLAS Technol
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So, for example, during expression and purification of a recombinant protein, it may have a disulfide bond form, which wouldnt normally form in the proteins natural context
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