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For example, immunohistochemical detection of MC 1 was shown in normal skin samples from healthy volunteers, locating in dermal and epidermal regions

0.2% in PBS, 10 minutes, room temperature) and blocked with donkey serum (5% in PBS, 1 hour, room temperature)

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The enzyme comprises two active regions: the C-terminal domain responsible for epoxide hydrolase activity and the N-terminal domain with phosphatase functionality, both of which are essential for the regulation of brain cholesterol metabolism and inflammatory responses [204]

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