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Just as described

Check for air bubbles in the syringe

G.et al (2000b)

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However, instead of relying on the biotinstreptavidin interaction, we developed an all-small molecule trifunctional tagging reagent containing an azide, a BODIPY fluorophore, and a methacrylamide unit for crosslinking to the hydrogel, in a method termed Lipid Expansion Microscopy (LExM) (Figure 78)