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The potency and duration of action of an N-(3-phenyl-1,2,4-thiadiazol-5-yl) piperazine analog, discovered through high-speed analog synthesis, were enhanced via structural optimization

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Notably, the 1,4-dihydropyridine ring oxidized analogue (KL7) completely lost the adjuvant activity, presumably due to altered electronic structure, reduced conformational flexibility, or lost hydrogen bonding capacity (Extended Data Fig

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