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The supernatants were used to extract RNA through a combined semi-automated method using a Quiagen Rneasy 96 QIAcube HT kit
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In addition, it has two deliberate structural modifications that researchers believe significantly enhance its physicochemical profile for research settings
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TNF- damages cardiomyocyte mitochondria by inhibiting glycolysis and reducing mitochondrial adenosine triphosphate (ATP) production through promoting oxidative and nitrosative stress, which can result in compromised cardiac systolic function and unfavorable cardiac remodeling [83, 84]
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