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The stimulated cells were fixed with 4% PFA in PBS for 30 min and stained with 0.2% crystal violet in 20% methanol

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High levels of sugar in the blood (hyperglycemia) and insulin deficiency or resistance can lead to increased oxidative stress

1997 Jun;37(6):383-5

It also increases collagen III for tissue flexibility and drives collagen IV production up by a remarkable 25.4-fold when combined with hyaluronic acid