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The cleared media was adjusted to a final concentration of 50 mM sodium/potassium phosphate at pH 6.5 and applied to the cation exchange column (Hitrap SP HP, Cytiva)

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PCR of wild type allele and targeted allele result in ~1500bp and ~800bp bands, respectively

[8] Their findings reveal important benefits for endurance athletes: [8] Blood flow was significantly augmented 30 minutes post-exercise in the L-citrulline + glutathione group compared to placebo