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2019;149:e59833
Interestingly, some JAK2-activating mutations including V617F have been shown to require interaction with a cytokine receptor for their activity, suggesting that these mutations only disrupt inhibition of the pseudokinase when the JAK2 is bound to a receptor (9093)
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These give more information than Uroflow testing, though are slightly more invasive in that they require placement of a catheter
Briefly, pancreatic tissue was digested in Geys balanced salt solution containing 0.75 mg/mL collagenase P (Sigma-Aldrich), 0.03% protease (Roche, Mannheim, Germany), and 0.01% DNase I (Roche)
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