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Nrf2 also induces glutathione-related genes, including GSTM1 and GCLM (encoding glutathione S-transferase 1 and glutamate-cysteine ligase modifier subunit, respectively), and matrix-assisted laser desorption/ionization-imaging mass spectrometry has demonstrated that compared to wild-type mice, glutathione is highly produced in the cortex and outer medulla in Keap1-KD mice before and after IRI [67]