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Following the latter protocol, we attained vacuum distillation of EBN, but failed to obtain a nitrone fraction that was free of trace amounts of nitroxides, which ultimately interfere with EPR spin-trapping experiments

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Specifically, the addition of cuprizone (bis-cyclohexanone oxalydihydrazone), to the diet process (66) produces a massive demyelination in different areas of the CNS and particularly in the corpus callosum (67-69)

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