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Our in vitro and in vivo studies proved that FreSHtracer is a useful tool for the real-time monitoring of cellular GSH levels in living MSCs and for isolating MSCs based on their GSH levels
B12) to a cocktail of supplements (i.e
The second order rate constant k ox for the nonenzymatic reaction between GSSG and roGFP2 WT (SH) 2 was 0.7 M 1 s 1 (Fig
5 Apoptotic death of the retinal ganglion cells (RGCs) has been shown 6 and, in addition, the effect of the intraocular hypertension causes a significant overexpression of caspase 3, which is a commonly accepted marker of programmed cell death
Cytochemical SA--galactosidase assay Cells were seeded in six-well plates, fixed with 0.5% glutaraldehyde (Sigma, G5882) in PBS for 15 min, washed with 1 mM MgCl 2 /PBS (pH 6.0) and then incubated with X-Gal staining solution (1 mg ml 1 of X-Gal (Thermo Scientific, 2737087), 5 mM K 3 (Fe(CN) 6 ) and 5 mM K 4 (Fe(CN) 6 ) for 68 h at 37 C
