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Bisulfite sequencing PCR To assess the impact of VCD and supplemental vitamin B12 on the methylation levels of 17 CpG sites located in the promoter region and proximal portion of the first exon of CCL11 , DNA libraries obtained from cultures of endpoints A and B were analyzed using Bisulfite Sequencing PCR (BSP)

Yes and B12 should be shipped with an ice pack to maintain temperature levels

Divide your target dose (mcg) by your reconstituted concentration (mcg/mL) to get the volume in mL