If you expect nothing, it might interpret normal sensations as side effects
Beyond discomfort, theyre also commonly accompanied by fatigue, dizziness, and nausea, all of which can make it more difficult to sustain longer or higher-intensity efforts
It is not widely utilized and therefore data is still limited
Flies were washed (five times for 3 min each) with 0.15 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 on ice, postfixed with 1% osmium tetroxide in 0.15 M sodium cacodylate buffer pH 7.4 for 1 h on ice, washed (five times for 3 min each) with ddH 2 O on ice and dehydrated in an ethanol series (20%, 50%, 70%, 90% and 100%) for 5 min each on ice, then 100% dry ethanol at room temperature twice for 5 min each, then 1:1 v/v dry ethanol and dry acetone for 5 min at room temperature and finally dry acetone for 5 min at room temperature
PKC is an activator of neuronal mitochondrial metabolism that mediates the spacing effect on memory consolidation
This eliminates skin-on-skin contact, which often causes rashes and discomfort
