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Non-specific binding was blocked for 1 h at room temperature in TBST with 5% low-fat milk, after which the membranes were incubated overnight at 4 C with anti-PCBP1 (A19276, ABclonal

However, excessive caffeine intake can lead to side effects like anxiety, sleep disturbances and jitters

Felson DT, Lawrence RC, Dieppe PA, Hirsch R, Helmick CG, Jordan JM, Kington RS, Lane NE, Nevitt MC, Zhang Y, Sowers M, McAlindon T, Spector TD, Poole AR, Yanovski SZ, Ateshian G, Sharma L, Buckwalter JA, Brandt KD, Fries JF (2000) Osteoarthritis: new insights

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The method of embodiment 83, wherein the internal radiation therapy ototoxicity is low dose rate internal radiation therapy