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Flow cytometry was performed on a LSRFortessa X-20 (Becton Dickinson, Franklin Lakes, NJ) and data were analyzed using FlowJo (v7.1.6, TreeStar)

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Loren Pickart's original 1973 identification of GHK characterized it as a factor in human plasma responsible for restoring proliferation to liver cells in cultures supplemented with older-donor plasma

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