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Most people will first notice that they start to have pain and stiffness, and this is often much worse at night-time

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ISK and KGN cells were grown in DMEM/F12 (Gibco, New York, USA) containing 10% FBS

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The major isoform, 23 kDa prolactin, acts via its membrane receptor, the prolactin receptor (PRL-R), which is a member of the haematopoietic cytokine superfamily and for which the mechanism of activation has been deciphered