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Raji cells were transiently reverse-transfected with siTNFR1 (50 nM), and the cells were cultured for 72 h

In rat medial collateral ligament injury models, the peptide enhanced ligament strength and reduced healing time

Tarpey et al., 2017)

Although linear disulfides are used as TrxR-responsive motifs, in vitro assays conducted under subphysiological GSH concentrations (10 M vs 110 mM intracellularly) can obscure TrxR-specific activation by competing thiols, compromising their ability to model native enzyme behavior 21,22

Our findings indicate that glucose metabolism should be followed up if long-term NAD-interventions are needed