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From the perspective of antioxidant activity, the overall effect of the SPLET mechanism is equivalent to that of the HAT mechanism in neutralizing free radicals

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Sample preparation (B) In an alternate protocol, the frozen liver tissue was directly lysed by probe sonication (2 10 pulse, 40% power output) in 4 M urea/DPBS and briefly cleared of debris by centrifugation (5000 g , 5 min, 4 C)

Subsequently, the sections were stained with hematoxylin and eosin (H&E) according to standard protocols