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S1A), the cell ROS dichlorofluorescein (DCF) assay in combination with flow cytometry analysis demonstrated that WBC ROS accumulations during 1 h of 2 g/kg glucose challenge in the LOsG group were significantly higher than in the sham group (Fig

Add the clear sample containing target GST-tagged protein onto the equilibrated gravity column, keep the sample in column for at least 2 min, ensure that the sample fully binds with the resin, and collect the flowthrough with a flow-rate of 0.5 - 1 mL per minute, then add the flowthrough onto the column again, repeat the previous operation, this can increase the binding efficiency

The impact of fermentation on bee pollen polyphenolic compounds composition

The BBD data were analyzed using contour and 3D plots to elucidate the independent variables' effects

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