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Expression and purification of SmTrx SmTrx was expressed, purified, and quantified using methods previously established ( Anaerobic techniques and transient-state conditions Following published protocols ( For each variant the reductive half-reaction was observed using both photomultiplier (PMT) detection at individual wavelengths specific to the observation and using charge coupled device (CCD) detection for all wavelengths between 300 and 800 nm
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One inpatient diagnosis, or 2 different outpatient encounter diagnoses were required to meet criteria for a respective event (see list in Supplementary Table S9).We extracted date of death from the VHA Death Ascertainment File which contain mortality data from the Master Person Index file in CDW and the Social Security Administration Death Master File
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Additionally, we note that the inclusion of CCNs would not align with the TiC provider identifier requirements
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