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Minor side effects such as injection site pain, redness, or temporary headache are common and usually self-limiting

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Inject water slowly down the vial wall, swirl never shake label every vial, refrigerate, and avoid temperature cycling

HEK293T cells were co-transfected with the shRNA plasmids and two helper plasmids, pCMVR8.91, and pMD.G, using the Lipofectamine 2000 reagent

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