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After staining, the samples were imaged using an inverted microscope (Nikon, Japan), and the areas of positive staining were quantified using ImageJ software (National Institutes of Health, USA, to determine the relative areas of calcium nodules, lipid droplets, and cartilage matrix

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The authors assessed the effect of formononetin on preventing mast cell degranulation in rat basophilic leukemia RBL-2H3 and human basophilic chronic myelogenous leukemia KU812 cells and saw reduced degranulation without any evident cytotoxicity [121]

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