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Topical treatments for dark spots over 55 Vitamin C as a tyrosinase inhibitor Vitamin C interacts with copper ions at the tyrosinase active site and inhibits the enzyme's action, reducing melanin formation [20]

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Preparation of Cochlear Explants After dissecting the organ of Corti, cochlear explants were established in DMEM containing 10% FBS and N2 supplement and allowed to acclimate overnight at 37 C