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Real-Time Quantitative Reverse Transcription PCR (qRTPCR) Analysis Total RNA from skin tissues was isolated using TRIzol reagent (Sigma, Milwaukee, WI, USA), and 1 g of total RNA was reverse-transcribed into cDNA

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This announcement is limited to operational and documentation improvements, and it is designed to support laboratory workflows that depend on consistent identifiers, consistent labeling language, and consistent recordkeeping across repeated experiments