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A full description of the methodology has been published previously

doi: 10.1089/met.2018.0105 7

Angew Chem Int Ed Eng 55:1247512478 Mauvais-Jarvis F (2011) Estrogen and androgen receptors: regulators of fuel homeostasis and emerging targets for diabetes and obesity

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When PNP was inhibited pharmacologically by the compound immucillin H (ImmH), intracellular cleavage of NR to NAM was prevented, and NAD + synthesis was potentiated from NR both in vitro and in vivo 25

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