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The following conditions were used for the thermal cycler (T100 Thermal Cycler, BIO-RAD, Hercules, CA, USA): 10 min at 25 C, 120 min at 37 C, 5 min at 85 C, and 10 min at 4 C

It is characterized by slow brain waves (0.5-4 Hz) and is associated with physical recovery, memory consolidation, and growth hormone (GH) secretion

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