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eWAT explants (500 mg) were digested in 1 mL DMEM supplemented with 2% fatty acidfree BSA (Sigma-Aldrich, 126575), HEPES (10 mM), Liberase TM (thermolysin medium) (25 g/mL) (Roche, 05401119001), and DNAse (250 g/mL) (Roche, 10104159001) for 1 hour at 37C

Furthermore, elevated hypothalamic NPY levels in CCK-B receptorknockout mice have been linked to hyperphagia, increased fat deposition, and obesity (33, 34)

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This has made it an attractive option for individuals with obesity or those struggling with weight management, even in the absence of diabetes

During DPP-4 inhibition, levels of active GLP-1 increase up to 2-3 times

These plans should be customized to match the individual's fitness level and physical capacity to ensure adherence and effectiveness [166,167]