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RESULTS Screening di-domain glycerol-3-phosphate dehydrogenases (GPDHs) for their ability to modulate cellular DHAP/Gro3P levels and NAD + recycling in mammalian cells To facilitate expression in HeLa cells under a doxycycline (Dox)-inducible promoter, constructs encoding four di-domain glycerol-3-phosphate dehydrogenases (GPDHs) from algae ( Dunaliella viridis , Dunaliella salina , Chlamydomonas reinhardtii and Sphaeroforma arctica ) were engineered through H