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The skin is a formidable barrier, and most peptides cannot cross it without chemical enhancers, microneedles, or iontophoresis (electrical current)

Journal of the American Medical Association, 280 (5), 443-448

Your veterinarian can provide guidance on the appropriate dosage and form of glutathione supplement for your dog, as well as monitor for any potential side effects or interactions with other medications

If you use sterile water, you should ideally use the entire vial in a single session