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The author GC declared that they were an editorial board member of Frontiers, at the time of submission

Neutrophils are signaled to the areas of inflammation even without infection present, but bacterial presence can push this neutrophilic response further, generating more oxidative stress from released reactive oxygen species, more crosslinking of the mucins from the oxidative stress, and more DNA released from dying epithelial and neutrophil cells (Yuan et al., 2015

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4 (4), e1368

Primary cells are another option which have much higher TEER and lower permeability than the available cell lines, but they are more time consuming, expensive, and can be passaged a limited number of times