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The fractions containing OsDHAR protein were pooled and concentrated using Amicon Ultra-15 Centrifugal Filters (Ultracel-10 K

Chondrocyte dedifferentiation was majorly characterized as the reduced expression of COL2 and ACAN and enhanced expression of type I collagen and versican ( COL2 expression, but not ACAN ( In addition to dedifferentiation, another consequence of in vitro expansion is replicative senescence, which also significantly influences chondrocyte function

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Statistical analyses were conducted using appropriate methods, and results were presented with 95% confidence intervals and corresponding p-values